Research-grade optics
High-resolution infinity optics and suitable high-NA objectives support detailed observation of cells, tissue sections, chromosomes and fluorescent biological targets.
Biological fluorescence microscope
LMI BM-R Series
A high-end modular research fluorescence microscope for demanding life-science imaging, including FISH, karyotyping, cytogenetics, immunofluorescence, cell biology, pathology and molecular biology workflows.
- FISH, chromosome imaging and cytogenetics configurations
- Multi-channel epi-fluorescence for DAPI, FITC, TRITC, Cy3 and Cy5
- 26.5 mm super-wide field of view
- Scientific monochrome or colour camera integration
- Motorized focus, XY stage, nosepiece and fluorescence selection options
Optical systemInfinity corrected
Field of viewUp to 26.5 mm
Fine focusUp to 0.001 mm
Research applicationsFISH & cytogenetics
Designed for research
Comfort and control in every detail.
The BM-R’s large, double-way linear-drive stage can be controlled from either side and supports two slides for comparative study. Its anti-corrosive, anti-friction surface and arc-transition platform help improve durability and protect against impact.
A modular frame separates the cross arm from the main body, improving compatibility between biological and fluorescence systems. Low-position coaxial controls, adjustable tension and an upper limit stop provide accurate focusing and comfortable extended use.
Multiple trinocular light-splitting options direct light to the eyepieces, camera, or both simultaneously. Inverted- and erect-image heads are available for observation, documentation and motorized workflows.
Advanced fluorescence imaging
Built for demanding life-science research
The BM-R can combine infinity-corrected research optics, high-numerical-aperture objectives, efficient epi-fluorescence illumination, precision mechanics and sensitive digital imaging. Each system is configured around the fluorophores, specimens, observation methods and automation requirements of the laboratory.
Multi-channel fluorescence
Filter configurations can support UV, blue, green, yellow, orange, red and far-red fluorescence according to the laboratory's fluorophores and application.
Sensitive digital imaging
Choose a monochrome scientific camera for demanding fluorescence detection or a colour camera for brightfield, histology and routine biological documentation.
Precision focusing
Manual or motorized focusing supports accurate work with thin specimens and can enable repeatable Z-stack acquisition with compatible imaging software.
Motorized automation
Optional motorized nosepiece, focus, XY stage, fluorescence selection and illumination control support multichannel, multi-position and automated imaging.
Expandable platform
Add fluorescence channels, objectives, scientific cameras, motorized components and analysis software as research requirements develop.
FISH and chromosome imaging
Configured for cytogenetics and fluorescent probes
Fluorescence In Situ Hybridization uses fluorescently labelled probes to identify DNA sequences within chromosomes or cells. A suitable BM-R configuration combines stable focusing, high-resolution optics, efficient illumination, accurate channel separation and a sensitive camera.
FISH workflow
- Locate the specimen under transmitted light
- Identify metaphase or target regions
- Select the required fluorescence channel
- Focus at the appropriate magnification
- Capture and document fluorescent signals
Recommended capabilities
- High-NA research objectives
- DAPI, FITC, TRITC, Cy3 or Cy5 filter sets
- Stable fine focusing
- Scientific monochrome camera
- Multichannel acquisition software
Advanced options
- 60X or 100X oil-immersion imaging
- Motorized XY stage and focusing
- Automated filter-cube selection
- Z-stack and multi-position acquisition
- Image analysis and channel merging
Fluorescence optical pathway
Efficient excitation and emitted-light detection
The epi-fluorescence system directs excitation light through the objective, separates excitation and emission wavelengths, and sends the fluorescent signal to the eyepiece or camera.
Excitation source
Excitation filter
Dichroic mirror & objective
Fluorescent specimen
Emission filter & camera
Fluorophores and applications
Filter sets selected for the laboratory workflow
Final excitation and emission specifications should always be matched to the actual fluorophores used by the laboratory.
| Fluorophore or method | Typical application |
|---|---|
| DAPI | Nuclear and DNA staining |
| FITC / GFP | Green fluorescence and fluorescent-protein imaging |
| TRITC / Cy3 | Red or orange fluorescence |
| Cy5 | Far-red fluorescence |
| Alexa Fluor dyes | Multicolour fluorescence |
| FISH probes | Chromosome and genetic analysis |
Research applications
One platform for multiple biological workflows
Cell & molecular biology
Observe labelled cells, proteins, nucleic acids, intracellular components and molecular targets.
Immunofluorescence
Image fluorescently labelled antibodies, cell markers and tissue targets with suitable filter sets.
Cytogenetics & FISH
Support chromosome imaging, fluorescent probes, karyotyping workflows and genetic analysis.
Histopathology
Combine conventional brightfield observation with fluorescence-based specimen examination.
Microbiology
Visualize microorganisms, cellular structures and fluorescent markers.
Biomedical research
Configure the optical platform for neuroscience, developmental biology and other research applications.
Technical information
BM-R specifications
Choose from manual, fluorescence, digital and motorized components to create the right configuration for your application.
Model
BM-R / BM-Prime / FLUO-Prime Series
Optical system
Infinity-corrected, anti-fungal optical system.
Eyepieces
PL10X/25 mm high eye-point, wide-field plan eyepiece with diopter adjustment; reticle version available.
PL10X/26.5 mm high eye-point, wide-field plan eyepiece with diopter adjustment; reticle version available.
Objectives
Plan semi-apochromatic fluorescence objectives: 4X, 10X, 20X, 40X and 100X.
Infinity plan achromatic objectives: 2X, 4X, 10X, 20X, 40X and 100X.
Infinity plan phase-contrast objectives: 10X, 20X, 40X and 100X.
Viewing head
LMI inverted-image 30°/25° inclined Siedentopf trinocular head; 50–76 mm interpupillary distance; R:T ratios of 100:0, 20:80 or 0:100.
Erect-image 30°/25° inclined Siedentopf trinocular head; 50–76 mm interpupillary distance; R:T ratios of 100:0 or 0:100.
Nosepiece
Revolving quadruple, quintuple, sextuple or septuple nosepiece, with optional DIC slot.
Body
Coaxial focusing system with upper limit and tension adjustment; 30 mm coarse range, 0.002 mm fine precision and adjustable focus height.
Stage
187 × 168 mm double-layer mechanical stage; 80 × 55 mm movement range; 0.1 mm precision; two-way linear drive with adjustable tension.
Optional 175 × 145 mm rotatable, anti-corrosive and anti-friction stage; right- or left-hand X/Y controls; 76 × 50 mm movement range.
Optional 187 × 166 mm stage with 80 × 50 mm movement range and 0.1 mm precision.
Frame
Biological transmitted-light frame with low-position coaxial coarse and fine adjustment; 25 mm coarse range and 0.001 mm fine precision.
Fluorescence transmitted-light frame with the same focus controls. Both versions include 100–240 V AC, 50/60 Hz wide-voltage transformer, digital intensity setting/reset and LBD, ND6 and ND25 filters.
Condenser
N.A. 0.9 or N.A. 1.2/0.22 swing-out achromatic condenser; N.A. 1.25 five-position phase-contrast condenser; N.A. 0.9 dry or N.A. 1.25 oil dark-field condenser.
LED fluorescence
UV2 long-pass LED module, 365 nm; B4 TB module, 455 nm; B1 band-pass module, 470 nm; G1 band-pass module, 560 nm.
Each module includes intensity control and bright-field/fluorescence switching. Additional modules can be tailored to clinical diagnostic requirements.
Mercury fluorescence
100 W, 120 W or 130 W mercury reflected fluorescence illuminator; optional 75 W metal-halide xenon lamp with approximately 2,000 hours of service life.
Transmitted illumination
100–240 V AC, 50/60 Hz input with 3 W high-brightness LED and continuous intensity adjustment.
Optional 6 V / 30 W halogen bulb with preset filament centre and continuous intensity adjustment.
Polarizing kit
360° rotatable analyzer; polarizer and analyzer can be moved out of the light path.
Filters
Yellow, green, blue and neutral filters.
Battery supply
Rechargeable external power supply providing at least eight hours of operation.
Light splitting
R:T 70:30 or 100:0 with special 1X CTV.
Camera adapters
0.5X CTV, 0.67X CTV and 1X CTV.